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Rabbit anti-CamK4 Antibody, Affinity Purified

Catalogue number:
A300-492A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to CamK4 immobilized on solid support. The epitope recognized by A300-492A maps to a region between residues 350 and 400 of human Calcium/Calmodulin-dependent Protein Kinase IV using the numbering given in Swiss-Prot entry Q16566 (GeneID 814). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human CamK4 by western blot
Product Type:

Antibodies

Alternative Names:

brain Ca(2+)-calmodulin-dependent protein kinase type IV, brain Ca++-calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase type IV catalytic chain, CAM kinase- GR, CAM

Species Reactivity:

Human

Immunogen:

between 350 and 400

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human CamK4 by western blot

Detection of human CamK4 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from Jurkat (5, 15 and 50 µg) and HeLa (50 µg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CamK4 antibody BL1834 (Cat. No. A300-492A) used at 0.1 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). CamK4 was also immunoprecipitated using CamK4 antibodies BL1835 (Cat. No. A300-493A) and BL1836 (Cat. No. A300-494A) used at 3 µg/mg lysate for IP. Detection: Chemiluminescence with an exposure time of 30 seconds.