Antibody was affinity purified using an epitope specific to Cytoskeletal Actin immobilized on solid support. The epitope recognized by A300-491A maps to the N-terminus of human Beta-actin using the numbering given in Swiss-Prot entry P60709 (GeneID 60). The N-terminus of Beta-Actin is highly conserved with Gamma-Actin and preliminary indications are that A300-491A also recognizes Gamma-Actin (GeneID 71). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
actin, cytoplasmic 1, beta cytoskeletal actin, Beta-actin, BRWS1, I(2)-actin, PS1TP5-binding protein 1, PS1TP5BP1
2 - 8°C
Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody A300-491A (lot A300-491A-17) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.