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Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified

Catalogue number:
A300-491A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Cytoskeletal Actin immobilized on solid support. The epitope recognized by A300-491A maps to the N-terminus of human Beta-actin using the numbering given in Swiss-Prot entry P60709 (GeneID 60). The N-terminus of Beta-Actin is highly conserved with Gamma-Actin and preliminary indications are that A300-491A also recognizes Gamma-Actin (GeneID 71). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

actin, cytoplasmic 1, beta cytoskeletal actin, Beta-actin, BRWS1, I(2)-actin, PS1TP5-binding protein 1, PS1TP5BP1

Species Reactivity:

Human, Mouse

Immunogen:

N-terminus

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse Cytoskeleta

Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody A300-491A (lot A300-491A-17) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human Cytoskeletal Actin by

Detection of human Cytoskeletal Actin by immunohistochemistry.  Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin (Cat. No. A300-491A lot 17) used at 1:5,000  (1µg/ml). Detection: DAB