Antibody was affinity purified using an epitope specific to CRM1 (Exportin 1) immobilized on solid support. The epitope recognized by A300-490A maps to a region between residues 375 and 425 of human Chromosome Region Maintenance 1 (Exportin 1) using the numbering given in entry NP_003391.1 (GeneID 7514). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
chromosome region maintenance 1 homolog, chromosome region maintenance 1 protein homolog, CRM1, emb, exp1, exportin 1 (CRM1 homolog, yeast), exportin-1, exportin-1 (required for chromosome region maintenance)
between 375 and 425
2 - 8°C
Detection of CRM1 (Exportin 1) by immunoprecipitation. Samples: Whole cell lysate (1 mg/IP; ¼ of reaction loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody BL1956 (Cat. No. A300-490A) used at 3 µg/mg lysate for IP. CRM1 was also immunoprecipitated by rabbit anti-CRM1 antibodies BL1958 (Cat. No. A300-469A) and less efficiently by BL1957, which recognize downstream epitopes on CRM1. immunoprecipitatesd CRM1 was blotted using BL1958 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.