Rabbit anti-ASH2 Antibody, Affinity Purified

Name: Rabbit anti-ASH2 Antibody, Affinity Purified
Brand: Bethyl Laboratories, Inc.
SKU: CBSKU_00070780
Price: 436 GBP
Availability: InStock

Catalogue number:

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A300-489A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µl (1000 µg/ml) _$$_

Product is available in:

Currency:
GBP
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£436.00

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Estimated delivery Wed 1 May
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Applications:

WB, IP, IHC, ChIP, ChIP-chip

Antibody Host:

Rabbit

Target:

ASH2

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 575 and C-term

Alternative Names:

ASH2; set1/Ash2 histone methyltransferase complex subunit ASH2; Bre2; ASH2-like protein; ASH2L2; ash2 (absent, small, or homeotic)-like; ASH2L1

Product Description:

Rabbit anti-ASH2 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human ASH2 by western blot and immunoprecipitation. Samples: A. Whole cell lysate from HEK293T cells that were mock transfected (E, 50 µg) or transfected with ASH2 expression constructs containing HA-tagged ASH2 (H, 25 µg) or Flag-tagged ASH2 (F, 25 µg). B. Whole cell lysate from one 10cm plate of normal 293T cells (~1 mg protein; 1/2 of IP loaded/lane). Antibodies: Affinity purified rabbit anti-ASH2 antibody A300-489A used at 1 µg/ml for WB (A and B) and at 5 µg/plate for IP. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody A300-107A using 5 µg/plate. Detection: Chemiluminescence with an exposure time of 1 second (A and B).

ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-489A was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitatesd DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.

Detection of human ASH2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 (Cat. No. A300-489A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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