Antibody was affinity purified using an epitope specific to CtIP (RBBP8) immobilized on solid support. The epitope recognized by A300-487A maps to a region between residues 525 and 575 of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COM1, CTBP-interacting protein, CTIP, DNA endonuclease RBBP8, JWDS, RBBP-8, retinoblastoma binding protein 8, Retinoblastoma-binding protein 8, Retinoblastoma-interacting protein and myosin-like, RIM, SAE2, SCKL2, sporulation in the absence of SPO11 prote
between 525 and 575
2 - 8°C
Detection of human CtIP by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (Cat. No. A300-487A) used at 3 µg/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (Cat. No. A300-488A) using 3 µg/mg lysate. immunoprecipitatesd CtIP was blotted using BL1914 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.