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Product types:
T-Cell Marker Antibodies
Bromodomain-Containing (BRD) Antibodies

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Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified - A300-485A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-485A
Name:
Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Cytoskeletal Actin immobilized on solid support. The epitope recognized by A300-485A maps to the N-terminus of human Gamma-actin using the numbering given in Swiss-Prot entry P63261 (GeneID 71). The N-terminus of Gamma-Actin is highly conserved with Beta-Actin and preliminary indications are that A300-485A also recognizes Beta-Actin (GeneID 60).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ACT, ACTG, actin, cytoplasmic 2, cytoskeletal gamma-actin, DFNA20, DFNA26, epididymis luminal protein 176, Gamma-actin, HEL-176
Species Reactivity:
Human, Mouse
Immunogen:
N-terminus
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse Actin by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (50µg), Jurkat (50µg), and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-Actin antibody A300-485A (lot A300-485A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Actin by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin (Cat. No. A300-485A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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