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Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified

Catalogue number:
A300-485A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Cytoskeletal Actin immobilized on solid support. The epitope recognized by A300-485A maps to the N-terminus of human Gamma-actin using the numbering given in Swiss-Prot entry P63261 (GeneID 71). The N-terminus of Gamma-Actin is highly conserved with Beta-Actin and preliminary indications are that A300-485A also recognizes Beta-Actin (GeneID 60). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

ACT, ACTG, actin, cytoplasmic 2, cytoskeletal gamma-actin, DFNA20, DFNA26, epididymis luminal protein 176, Gamma-actin, HEL-176

Species Reactivity:

Human, Mouse

Immunogen:

N-terminus

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse Actin by we

Detection of human and mouse Actin by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (50µg), Jurkat (50µg), and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-Actin antibody A300-485A (lot A300-485A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Actin by immunohistoc

Detection of human Actin by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin (Cat. No. A300-485A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB