Antibody was affinity purified using an epitope specific to Cytoskeletal Actin immobilized on solid support. The epitope recognized by A300-485A maps to the N-terminus of human Gamma-actin using the numbering given in Swiss-Prot entry P63261 (GeneID 71). The N-terminus of Gamma-Actin is highly conserved with Beta-Actin and preliminary indications are that A300-485A also recognizes Beta-Actin (GeneID 60). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ACT, ACTG, actin, cytoplasmic 2, cytoskeletal gamma-actin, DFNA20, DFNA26, epididymis luminal protein 176, Gamma-actin, HEL-176
2 - 8°C
Detection of human and mouse Actin by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (50µg), Jurkat (50µg), and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-Actin antibody A300-485A (lot A300-485A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.