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Rabbit anti-RCH1/KPNA2 Antibody, Affinity Purified - A300-484A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RCH1/KPNA2 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-484A
Name:
Rabbit anti-RCH1/KPNA2 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to RCH1/KPNA2 immobilized on solid support. The epitope recognized by A300-484A maps to a region between residue 475 and the C-terminus (residue 529) of human RAG cohort 1 (Karyopherin Alpha 2) using the numbering given in entry NP_002257.1 (GeneID 3838).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
importin subunit alpha-1, importin subunit alpha-2, importin-alpha-P1, IPOA1, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), Karyopherin subunit alpha-2, pendulin, QIP2, RAG cohort protein 1, RCH1, SRP1alpha, SRP1-alpha
Species Reactivity:
Human, Mouse
Immunogen:
Between 475 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse RCH1/KPNA2 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg), HEK293T (50µg), and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody A300-484A (lot A300-484A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody A300-484A (lot A300-484A-2) used for IP at 6 µg per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot A300-484A-1) and rabbit anti-RCH1/KPNA2 antibody A300-483A-2. For blotting immunoprecipitated RCH1/KPNA2, A300-484A was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human RCH1 by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- RCH1 (Cat. No. A300-484A Lot2) used at a dilution of 1:1,000 (1µg/ml) and 1:5,000 (0.2µg/ml). Detection: DAB

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