Antibody was affinity purified using an epitope specific to RCH1/KPNA2 immobilized on solid support. The epitope recognized by A300-483A maps to a region between residues 1 and 50 of human RAG cohort 1 (Karyopherin Alpha 2) using the numbering given in entry NP_002257.1 (GeneID 3838). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
importin subunit alpha-1, importin subunit alpha-2, importin-alpha-P1, IPOA1, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), Karyopherin subunit alpha-2, pendulin, QIP2, RAG cohort protein 1, RCH1, SRP1alpha, SRP1-alpha
between 1 and 50
2 - 8°C
Detection of human RCH1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RCH1 antibody A300-483A (lot number A300-483A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RCH1 was also immunoprecipitated by a previous lot of A300-483A (lot number A300-483A-1) and by rabbit anti-RCH1 antibody A300-484A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).