Antibody was affinity purified using an epitope specific to DDX3 immobilized on solid support. The epitope recognized by A300-474A maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 using the numbering given in entry NP_001347.2 (GeneID 1654). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DDX3X, CAP-Rf, DBX, DDX14, DDX3, DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, DEAD/H (Asp-Glu-Ala-Asp/His) box polyp
between 1 and 50
2 - 8°C
Detection of human DDX3 by western blot and immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 µg) and Ramos (50 µg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX3 antibody BL1649 (Cat. No. A300-474A) used at 0.04 µg/ml (A) and 0.1 µg/ml (B) for western blot and at 3 µg/mg lysate for IP (B). DDX3 was also immunoprecipitated using rabbit anti-DDX3 antibodies BL1650 (Cat. No. A300-475A) and BL1651 (Cat. No. A300-476A) at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).
Detection of human and mouse DDX3 by immunohistochemistry. Sample: FFPE section of human lung cancer pleural effusion (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX3 (Cat. No. A300-474A) used at a dilution of 1:200 (1µg/ml). Detection: Red fluorescence and DAB.