Antibody was affinity purified using an epitope specific to Alpha-4 (IGBP1) immobilized on solid support. The epitope recognized by A300-470A maps to a region between residue 300 and the C-terminus (residue 339) of human Protein Phosphatase 2A, regulatory subunit alpha-4 (Immunoglobulin binding protein 1) using the numbering given in SwissProt entry P78318 (GeneID 3476). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ALPHA-4, B cell signal transduction molecule alpha 4, bA351K23.1 (immunoglobulin binding protein 1 (CD79A) ), B-cell signal transduction molecule alpha 4, CD79a-binding protein 1, IBP1, immunoglobulin (CD79A) binding protein 1, immunoglobulin-binding prot
between 300 and C-term
2 - 8°C
Detection of Alpha 4 by western blot and immunoprecipitation. Samples: A. Whole cell lysate (50 µg) from Ramos (R), K-562 (K) and mouse NIH 3T3 (M) cells. B. Whole cell lysate (1 mg/IP; ¼ of IP reaction loaded/lane) from Ramos cells. Antibodies: Affinity purified rabbit anti-Alpha 4 antibody BL1846 (Cat. No. A300-471A) used at 0.4 µg/ml (A) and 1 µg/ml (B) for WB and at 3 µg/mg lysate for IP. Alpha 4 was also immunoprecipitated by BL1845 (Cat. No. A300-470A), but not by BL1844, which are affinity purified antibodies to upstream epitopes on Alpha 4. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).