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Rabbit anti-CRM1 Antibody, Affinity Purified - A300-469A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-CRM1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-469A
Name:
Rabbit anti-CRM1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to CRM1 (Exportin 1) immobilized on solid support. The epitope recognized by A300-469A maps to a region between residue 1025 and the C-terminus (residue 1071) of human Chromosome Region Maintenance 1 (Exportin 1) using the numbering given in entry NP_003391.1 (GeneID 7514).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
chromosome region maintenance 1 homolog, chromosome region maintenance 1 protein homolog, CRM1, emb, exp1, exportin 1 (CRM1 homolog, yeast), exportin-1, exportin-1 (required for chromosome region maintenance)
Species Reactivity:
Human, Mouse
Immunogen:
Between 1025 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse CRM1 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody A300-469A (lot A300-469A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human CRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa and cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody A300-469A (lot A300-469A-2) used for IP at 6 µg per reaction. CRM1 was also immunoprecipitated by rabbit anti-CRM1 antibody A300-490A. For blotting immunoprecipitated CRM1, A300-469A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human CRM1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 (Cat. No. A300-469A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

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Detection of mouse CRM1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 (Cat. No. A300-469A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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