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Rabbit anti-MST1-2/STK3-4 Antibody, Affinity Purified - A300-466A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MST1-2/STK3-4 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-466A
Name:
Rabbit anti-MST1-2/STK3-4 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MST1 and MST2 immobilized on solid support. The epitope recognized by A300-466A maps to the C-terminus of human Mammalian Sterile 20-like 1 (Serine/Threonine Kinase 4) using the numbering given in Swiss Prot entry Q13043 (GeneID 6789). The epitope is highly conserved with MST2/STK3. Consequently, A300-466A also recognizes this homolog.Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
kinase responsive to stress 2, KRS2, mammalian STE20-like protein kinase 1, mammalian sterile 20-like 1, MST1, MST-1, serine/threonine-protein kinase 4, serine/threonine-protein kinase Krs-2, STE20-like kinase MST1, YSK3
Species Reactivity:
Human, Mouse
Immunogen:
C-terminus
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of MST1 and MST2 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 µg), Ramos (R, 50 µg) and mouse NIH 3T3 (M, 50 µg) cells. B) Whole cell lysate (1 mg/IP reaction; 1/4 of reaction loaded/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-MST1/2 antibody A300-466A used at 0.1 µg/ml for WB (A and B) and 3 µg/mg lysate for IP (B). MST1 was immunoprecipitated using MST1 antibody A300-465A. MST2 was immunoprecipitated using MST2 antibody A300-467A. MST1 and MST2 were simultaneously immunoprecipitated using MST1/2 antibodies A300-466A and A300-468A. Detection: Chemiluminescence with an exposure times of 3 seconds (A and B). A300-466A recognized MST1 in IP from A300-465A and MST2 in IP from A300-467A.

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Detection of human MST1 and MST2 by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti- MST1/2 (Cat. No. A300-466A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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