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Rabbit anti-HDAC3 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Fri 5 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 375 and C-term

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Product Description:

Rabbit anti-HDAC3 Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse HDAC3 by we
Detection of human and mouse HDAC3 by western blot and human HDAC3 by immunoprecipitation. Samples: A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 µg), Ramos (R; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody BL2127 (Cat. No. A300-464A) used at 0.02 µg/ml for WB (A) and at 3 µg/mg lysate for IP (B). immunoprecipitation reactions were also performed with anti-HDAC3 antibody BL2125 (to an upstream epitope) and BL2128 (to a downstream epitope) and normal rabbit IgG. HDAC3 in IP from BL2127 was blotted using BL2125 and BL2128 at 1 µg/ml. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human HDAC3 by immunohistoc
Detection of human HDAC3 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC3 (Cat. No. A300-464A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB
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