Antibody was affinity purified using an epitope specific to HDAC3 immobilized on solid support. The epitope recognized by A300-464A maps to a region between residue 375 and the C-terminus (residue 428) of human Histone Deacetylase 3 using the numbering given in Swiss-Prot entry O15379 (GeneID 8841). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HD3, histone deacetylase 3, RPD3, RPD3-2, SMAP45
between 375 and C-term
2 - 8°C
Detection of human and mouse HDAC3 by western blot and human HDAC3 by immunoprecipitation. Samples: A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 µg), Ramos (R; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody BL2127 (Cat. No. A300-464A) used at 0.02 µg/ml for WB (A) and at 3 µg/mg lysate for IP (B). immunoprecipitation reactions were also performed with anti-HDAC3 antibody BL2125 (to an upstream epitope) and BL2128 (to a downstream epitope) and normal rabbit IgG. HDAC3 in IP from BL2127 was blotted using BL2125 and BL2128 at 1 µg/ml. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human HDAC3 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC3 (Cat. No. A300-464A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB