Antibody was affinity purified using an epitope specific to DDB1 immobilized on solid support. The epitope recognized by A300-462A maps to a region between residue 1100 and the C-terminus (residue 1140) of human damage-specific DNA binding protein 1 using the numbering given in TrEMBL entry Q13289 (GeneID 1642). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
damage-specific DNA binding protein 1, 127kDa, Damage-specific DNA-binding protein 1, DDB p127 subunit, DDBA, DNA damage-binding protein 1, DNA damage-binding protein a, HBV X-associated protein 1, UV-damaged DNA-binding factor, UV-damaged DNA-binding pro
between 1100 and C-term
2 - 8°C
Detection of human and mouse DDB1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDB1 antibody A300-462A (lot A300-462A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human DDB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDB1 antibody A300-462A (lot A300-462A-3) used for IP at 3 µg per reaction. DDB1 was also immunoprecipitated by rabbit anti-DDB1 antibody BL1996. For blotting immunoprecipitated DDB1, A300-462A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.