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Rabbit anti-DDX6 Antibody, Affinity Purified - A300-461A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DDX6 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-461A
Name:
Rabbit anti-DDX6 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to DDX6 immobilized on solid support. The epitope recognized by A300-461A maps to a region between residues 425 and 483 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry NP_004388.2 (GeneID 1656).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box helicase 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), HLR2, oncogene RCK, P54, probable ATP-dep
Species Reactivity:
Human, Mouse
Immunogen:
Between 425 and 483
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse DDX6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody A300-461A (lot A300-461A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human DDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX6 antibody A300-461A (lot A300-461A-3) used for IP at 6 µg per reaction. DDX6 was also immunoprecipitated by a previous lot of this antibody (A300-461A-2) and rabbit anti-DDX6 antibody A300-460A. For blotting immunoprecipitated DDX6, A300-461A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human DDX6 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- DDX6 Cat. No. A300-461A Lot 2 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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