Antibody was affinity purified using an epitope specific to DDX6 immobilized on solid support. The epitope recognized by A300-460A maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box helicase 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), HLR2, oncogene RCK, P54, probable ATP-dep
between 1 and 50
2 - 8°C
Detection of human DDX6 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB in A; 1 mg for IP, 1/4 of IP loaded in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody BL2139 (Cat. No. A300-460A) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody BL2142 (Cat. No. A300-461A), but not with BL2141, which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
Detection of human and mouse DDX6 by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX6 (Cat. No. A300-460A) used at a dilution of 1:200 (1µg/ml). Detection: DAB.