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Rabbit anti-ROCK1 Antibody, Affinity Purified - A300-457A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ROCK1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-457A
Name:
Rabbit anti-ROCK1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to ROCK1 immobilized on solid support.The epitope recognized by A300-457A maps to a region between residue 1300 and the C-terminus (residue 1354) of human Rho-associated, coiled-coil containing protein kinase 1 using the numbering given in Swiss-Prot entry Q13464 (GeneID 6093).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
p160 ROCK-1, P160ROCK, renal carcinoma antigen NY-REN-35, rho-associated protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I
Species Reactivity:
Human, Mouse
Immunogen:
Between 1300 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody A300-457A (lot A300-457A-2) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot A300-457A-2) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

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