Antibody was affinity purified using an epitope specific to ROCK1 immobilized on solid support. The epitope recognized by A300-455A maps to a region between residues 400 and 450 of human Rho-associated, coiled-coil containing protein kinase 1 using the numbering given in Swiss-Prot entry Q13464 (GeneID 6093). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
p160 ROCK-1, P160ROCK, renal carcinoma antigen NY-REN-35, rho-associated protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I
between 400 and 450
2 - 8°C
Detection of human ROCK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibodies BL2135, BL2136 (Cat. No. A300-455A), BL2137 (Cat. No. A300-456A) and BL2138 (A300-457A) used at 3 µg/mg lysate for IP. Normal rabbit IgG was used as a control for immunoprecipitation. ROCK1 was blotted with BL2135, BL2137, or BL2138 (each at 1 µg/ml). Detection: Chemiluminescence with exposure times of 10 to 30 seconds.