Antibody was affinity purified using an epitope specific to FOXO3a immobilized on solid support. The epitope recognized by A300-454A maps to a region between residue 625 and the C-terminus (residue 673) of human Forkhead Box O3a using the numbering given in Swiss-Prot entry O43524 (GeneID 2309). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3A, forkhead box protein O3, forkhead homolog (rhabdomyosarcoma) like 1, forkhead in rhabdomyosarcoma-like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, FOXO2, FOXO3A
between 625 and C-term
2 - 8°C
Detection of human and mouse FOXO3a by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody A300-454A (lot A300-454A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody A300-454A (lot A300-454A-2) used for IP at 6 µg/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibody A300-453A. For blotting immunoprecipitated FOXO3a, A300-454A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.