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Rabbit anti-FOXO3a Antibody, Affinity Purified - A300-454A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-FOXO3a Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-454A
Name:
Rabbit anti-FOXO3a Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to FOXO3a immobilized on solid support. The epitope recognized by A300-454A maps to a region between residue 625 and the C-terminus (residue 673) of human Forkhead Box O3a using the numbering given in Swiss-Prot entry O43524 (GeneID 2309).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3A, forkhead box protein O3, forkhead homolog (rhabdomyosarcoma) like 1, forkhead in rhabdomyosarcoma-like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, FOXO2, FOXO3A
Species Reactivity:
Human, Mouse
Immunogen:
Between 625 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse FOXO3a by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody A300-454A (lot A300-454A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody A300-454A (lot A300-454A-2) used for IP at 6 µg/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibody A300-453A. For blotting immunoprecipitated FOXO3a, A300-454A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human and mouse FOXO3a by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FOXO3a (Cat. No. A300-454A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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