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Rabbit anti-FOXO3a Antibody, Affinity Purified

Catalogue number:
A300-453A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to FOXO3a immobilized on solid support. The epitope recognized by A300-453A maps to a region between residues 250 and 300 of human Forkhead Box O3a using the numbering given in Swiss-Prot entry O43524 (GeneID 2309). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3A, forkhead box protein O3, forkhead homolog (rhabdomyosarcoma) like 1, forkhead in rhabdomyosarcoma-like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, FOXO2, FOXO3A

Species Reactivity:

Human, Mouse

Immunogen:

between 250 and 300

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse FOXO3a by w

Detection of human and mouse FOXO3a by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody A300-453A (lot A300-453A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human FOXO3a by western blo

Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody A300-453A (lot A300-453A-2) used for IP at 6 µg/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibody A300-454A. For blotting immunoprecipitated FOXO3a, A300-453A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human and mouse FOXO3a by i

Detection of human and mouse FOXO3a by immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FOXO3a (Cat. No. A300-453A Lot2) used at a dilution of 1:500 (2µg/ml). Detection: DAB