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High Quality Recombinant Rabbit Monoclonal Antibodies
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Rabbit anti-MSH2 Antibody, Affinity Purified - A300-451A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MSH2 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-451A
Name:
Rabbit anti-MSH2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MSH2 immobilized on solid support. The epitope recognized by A300-451A maps to a region between residues 1 and 50 of human mutS homolog 2 using the numbering given in Swiss-Prot entry P43246 (GeneID 4436).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
COCA1, DNA mismatch repair protein Msh2, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, mutS homolog 2, colon cancer, nonpolyposis type 1, MutS protein homolog 2
Species Reactivity:
Human, Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse MSH2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH2 antibody A300-451A (lot A300-451A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human MSH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MSH2 antibody A300-451A (lot A300-451A- 2 ) used for IP at 6 µg per reaction. MSH2 was also immunoprecipitated by a previous lot of this antibody (A300-451A-1) and rabbit anti-MSH2 antibody A300-452A. For blotting immunoprecipitated MSH2, A300-451A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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