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Rabbit anti-RecQ1 Antibody, Affinity Purified

Catalogue number:
A300-450A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to RecQ1 immobilized on solid support. The epitope recognized by A300-450A maps to a region between residue 600 and the C-terminus (residue 649) of human RecQ protein-like (DNA helicase Q1-like) 1 using the numbering given in Swiss-Prot entry P46063 (GeneID 5965). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

ATP-dependent DNA helicase Q1, DNA helicase, RecQ-like type 1, DNA-dependent ATPase Q1, RecQ helicase-like, RecQ protein-like (DNA helicase Q1-like), recQ protein-like 1, RecQ1, RECQL1

Species Reactivity:

Human

Immunogen:

between 600 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human RecQ1 by western blot

Detection of human RecQ1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RecQ1 antibody A300-450A (lot A300-450A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RecQ1 by western blot

Detection of human RecQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RecQ1 antibody A300-450A (lot A300-450A-2) used for IP at 6 µg per reaction. RecQ1 was also immunoprecipitated by rabbit anti-RecQ1 antibody A300-447A. For blotting immunoprecipitated RecQ1, A300-450A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RecQ1 by immunohistoc

Detection of human RecQ1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RecQ1 (Cat. No. A300-450A Lot2) used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB