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Rabbit anti-RecQ1 Antibody, Affinity Purified - A300-450A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RecQ1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-450A
Name:
Rabbit anti-RecQ1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to RecQ1 immobilized on solid support. The epitope recognized by A300-450A maps to a region between residue 600 and the C-terminus (residue 649) of human RecQ protein-like (DNA helicase Q1-like) 1 using the numbering given in Swiss-Prot entry P46063 (GeneID 5965).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ATP-dependent DNA helicase Q1, DNA helicase, RecQ-like type 1, DNA-dependent ATPase Q1, RecQ helicase-like, RecQ protein-like (DNA helicase Q1-like), recQ protein-like 1, RecQ1, RECQL1
Species Reactivity:
Human
Immunogen:
Between 600 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human RecQ1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RecQ1 antibody A300-450A (lot A300-450A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human RecQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RecQ1 antibody A300-450A (lot A300-450A-2) used for IP at 6 µg per reaction. RecQ1 was also immunoprecipitated by rabbit anti-RecQ1 antibody A300-447A. For blotting immunoprecipitated RecQ1, A300-450A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human RecQ1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RecQ1 (Cat. No. A300-450A Lot2) used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB

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