Antibody was affinity purified using an epitope specific to MnSOD immobilized on solid support. The epitope recognized by A300-449A maps to a region between residue 175 and the C-terminus (residue 222) of human Manganese Superoxide Dismutase using the numbering given in entry NP_000627.2 (GeneID 6648). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory sperm binding protein, indophenoloxidase B, IPOB, IPO-B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, Mn superoxide dismutase, MNSOD, Mn-SOD, MVCD6, superoxide dismutase [Mn], mitochondrial, superoxide dism
between 175 and C-term
2 - 8°C
Detection of MnSOD by western blot. Samples: Whole cell lysate (30 µg) from rat vascular smooth muscle cells treated with IL-1 beta (3 ng/ml) for 24 hours (+) or untreated (-). Antibody: Affinity purified goat anti-MnSOD (BL468G; Cat. No. A300-449A) used at 0.4 µg/ml. Detection: Chemiluminescence with 3 minute exposure.