Antibody was affinity purified using an epitope specific to RecQ1 immobilized on solid support. The epitope recognized by A300-447A maps to a region between residues 1 and 50 of human RecQ protein-like (DNA helicase Q1-like) 1 using the numbering given in Swiss-Prot entry P46063 (GeneID 5965). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent DNA helicase Q1, DNA helicase, RecQ-like type 1, DNA-dependent ATPase Q1, RecQ helicase-like, RecQ protein-like (DNA helicase Q1-like), recQ protein-like 1, RecQ1, RECQL1
between 1 and 50
2 - 8°C
Detection of human and mouse RecQ1 by western blot and human ReqQ1 by immunoprecipitation. Samples: A) Whole cell lysate (5, 15 and 50 µg) from human (HeLa) and mouse (NIH 3T3) cells. B) Whole cell lysate (1 mg) from HeLa cells. Antibodies: Affinity purified rabbit anti-RecQ1 antibody BL2071 (Cat. No. A300-447A) used at 0.1 µg/ml (A) and 1 µg/ml (B) for WB and at 3 µg/mg lysate for IP (B). RecQ1 was successfully immunoprecipitated using rabbit anti-RecQ1 antibody BL2074 (Cat. No. A300-450A) at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of two seconds (A and B).