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Rabbit anti-RecQ1 Antibody, Affinity Purified

Catalogue number:
A300-447A
Size:
20 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to RecQ1 immobilized on solid support. The epitope recognized by A300-447A maps to a region between residues 1 and 50 of human RecQ protein-like (DNA helicase Q1-like) 1 using the numbering given in Swiss-Prot entry P46063 (GeneID 5965). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

ATP-dependent DNA helicase Q1, DNA helicase, RecQ-like type 1, DNA-dependent ATPase Q1, RecQ helicase-like, RecQ protein-like (DNA helicase Q1-like), recQ protein-like 1, RecQ1, RECQL1

Species Reactivity:

Human, Mouse

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse RecQ1 by we

Detection of human and mouse RecQ1 by western blot and human ReqQ1 by immunoprecipitation. Samples: A) Whole cell lysate (5, 15 and 50 µg) from human (HeLa) and mouse (NIH 3T3) cells. B) Whole cell lysate (1 mg) from HeLa cells. Antibodies: Affinity purified rabbit anti-RecQ1 antibody BL2071 (Cat. No. A300-447A) used at 0.1 µg/ml (A) and 1 µg/ml (B) for WB and at 3 µg/mg lysate for IP (B). RecQ1 was successfully immunoprecipitated using rabbit anti-RecQ1 antibody BL2074 (Cat. No. A300-450A) at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of two seconds (A and B).

Detection of human RecQ1 by immunohistoc

Detection of human RecQ1 by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti- RecQ1 (Cat. No. A300-447A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB