www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-WSTF Antibody, Affinity Purified

Catalogue number:
A300-446A
Size:
20 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to WSTF immobilized on solid support. The epitope recognized by A300-446A maps to a region between residues 1 and 50 of human Williams Syndrome Transcription Factor using the numbering given in SwissProt entry Q9UIG0 (GeneID 9031). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

Bromodomain adjacent to zinc finger domain protein 1B, hWALp2, transcription factor WSTF, tyrosine-protein kinase BAZ1B, WBSCR10, WBSCR9, williams syndrome transcription factor, williams-Beuren syndrome chromosomal region 10 protein, williams-Beuren syndr

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human WSTF by western blot.

Detection of human WSTF by western blot. Samples: Whole cell lysate (50 µg) from HCT 116, HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WSTF antibody A300-446A (lot A300-446A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human WSTF by western blot

Detection of human WSTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WSTF antibody A300-446A (lot A300-446A-2) used for IP at 3 µg per reaction. WSTF was also immunoprecipitated by a previous lot of this antibody (lot A300-446A-1). For blotting immunoprecipitated WSTF, A300-446A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human WSTF by immunohistoch

Detection of human WSTF by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-WSTF (Cat. No. A300-446A Lot 2) used at a dilution of 1:200 (1µg/ml). Detection: DAB