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Rabbit anti-Paxillin Antibody, Affinity Purified - A300-444A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Paxillin Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-444A
Name:
Rabbit anti-Paxillin Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Paxillin immobilized on solid support. The epitope recognized by A300-444A maps to a region between residues 1 and 50 of human Paxillin using the numbering given in Swiss-Prot entry P49023 (GeneID 5829).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
paxillin, testicular tissue protein Li 134
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Paxillin by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-Paxillin antibody A300-444A (lot A300-444A-1) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). Paxillin was also immunoprecipitated with rabbit anti-Paxillin antibody BL866 using 3 µg/mg lysate. immunoprecipitation reactions were controlled using normal rabbit IgG (Ctrl IgG). Detection: Chemiluminescence with exposure times of 10 seconds.

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Detection of human Paxillin by immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti- Paxillin (Cat. No. A300-444A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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