Antibody was affinity purified using an epitope specific to Paxillin immobilized on solid support. The epitope recognized by A300-444A maps to a region between residues 1 and 50 of human Paxillin using the numbering given in Swiss-Prot entry P49023 (GeneID 5829). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
paxillin, testicular tissue protein Li 134
between 1 and 50
2 - 8°C
Detection of human Paxillin by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-Paxillin antibody A300-444A (lot A300-444A-1) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). Paxillin was also immunoprecipitated with rabbit anti-Paxillin antibody BL866 using 3 µg/mg lysate. immunoprecipitation reactions were controlled using normal rabbit IgG (Ctrl IgG). Detection: Chemiluminescence with exposure times of 10 seconds.