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Rabbit anti-PNUTS Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 3 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

PNUTS

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 900 and C-term

Alternative Names:

protein phosphatase 1, regulatory (inhibitor) subunit 10; testis tissue sperm-binding protein Li 67n; R111; Protein FB19; PP1R10; PP1-binding protein of 114 kDa; phosphatase 1 nuclear targeting subunit; p99; MHC class I region proline-rich protein CAT53;

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Product Description:

Rabbit anti-PNUTS Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human PNUTS by western blot
Detection of human PNUTS by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody A300-440A (lot A300-440A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PNUTS by western blot
Detection of human PNUTS by western blot and immunoprecipitation. Samples: Nuclear extract (NE; 15 and 45 µg for WB; 100 µg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-PNUTS antibody A300-440A (lot A300-440A-1) used at 1 and 0.2 µg/ml for WB (A) and at 1 µg/100 µg NE for IP (B). immunoprecipitation of PNUTS was also performed with rabbit anti-PNUTS antibodies BL1929, BL1930 and A300-439A. immunoprecipitatesd PNUTS was blotted using BL1932 at 0.2 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
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