Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support. The epitope recognized by A300-439A maps to a region between residues 350 and 400 of human Phosphatase Nuclear Targeting Subunit (a.k.a. Protein Phosphatase 1, regulatory subunit 10) using the numbering given in TrEMBL entry Q96QC0 (GeneID 5514). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAT53, FB19, HLA-C associated transcript 53, MHC class I region proline-rich protein CAT53, p99, phosphatase 1 nuclear targeting subunit, PNUTS, PP1-binding protein of 114 kDa, PP1R10, Protein FB19, protein phosphatase 1, regulatory (inhibitor) subunit 10
between 350 and 400
2 - 8°C
Detection of human PNUTS by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody A300-439A (lot A300-439A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PNUTS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PNUTS antibody A300-439A (lot A300-439A-3) used for IP at 3 µg per reaction. PNUTS was also immunoprecipitated by a previous lot of this antibody (lot A300-439A-2) and rabbit anti-PNUTS antibody A300-440A. For blotting immunoprecipitated PNUTS, A300-439A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.