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Rabbit anti-PNUTS Antibody, Affinity Purified

Catalogue number:
A300-439A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support. The epitope recognized by A300-439A maps to a region between residues 350 and 400 of human Phosphatase Nuclear Targeting Subunit (a.k.a. Protein Phosphatase 1, regulatory subunit 10) using the numbering given in TrEMBL entry Q96QC0 (GeneID 5514). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

CAT53, FB19, HLA-C associated transcript 53, MHC class I region proline-rich protein CAT53, p99, phosphatase 1 nuclear targeting subunit, PNUTS, PP1-binding protein of 114 kDa, PP1R10, Protein FB19, protein phosphatase 1, regulatory (inhibitor) subunit 10

Species Reactivity:

Human

Immunogen:

between 350 and 400

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human PNUTS by western blot

Detection of human PNUTS by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody A300-439A (lot A300-439A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PNUTS by western blot

Detection of human PNUTS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PNUTS antibody A300-439A (lot A300-439A-3) used for IP at 3 µg per reaction. PNUTS was also immunoprecipitated by a previous lot of this antibody (lot A300-439A-2) and rabbit anti-PNUTS antibody A300-440A. For blotting immunoprecipitated PNUTS, A300-439A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human PNUTS by immunohistoc

Detection of human PNUTS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PNUTS (Cat. No. A300-439A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB