Antibody was affinity purified using an epitope specific to CCAR1 immobilized on solid support. The epitope recognized by A300-435A maps to a region between residues 1 and 50 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in TrEMBL entry Q6X935 (GeneID 55749). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CARP-1, cell cycle and apoptosis regulatory protein 1, cell division cycle and apoptosis regulator protein 1, death inducer with SAP domain
between 1 and 50
2 - 8°C
Detection of human and mouse CCAR1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody A300-435A (lot A300-435A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CCAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody A300-435A (lot A300-435A-2) used for IP at 6 µg/mg lysate. CCAR1 was also immunoprecipitated by a previous lot (lot A300-435A-1) of the antibody. For blotting immunoprecipitated CCAR1, A300-435A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human and mouse CCAR1 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-CCAR1 (Cat. No. A300-435A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB