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Bromodomain-Containing (BRD) Antibodies

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Rabbit anti-DBC1/p30 DBC Antibody, Affinity Purified - A300-434A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DBC1/p30 DBC Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-434A
Name:
Rabbit anti-DBC1/p30 DBC Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DBC1 immobilized on solid support. The epitope recognized by A300-433A maps to a region between residue 875 and the C-terminus (residue 923) of human Deleted in Breast Cancer 1 using the numbering given in TrEMBL entry Q6P0Q9 (GeneID 57805).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, DBC.1, DBC1, DBC-1, DBIRD complex subunit KIAA1967, deleted in breast cancer 1, Deleted in breast cancer gene 1 protein, KIAA1967, NET35, p30 DBC, p30 DBC
Species Reactivity:
Human, Mouse
Immunogen:
Between 875 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human DBC1/p30 DBC by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DBC1/p30 DBC antibody A300-434A (lot number A300-434A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. DBC1/p30 DBC was also immunoprecipitated by a previous lot (A300-434A-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

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Detection of mouse DBC1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-DBC1 antibody A300-434A (lot A300-434A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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