Antibody was affinity purified using an epitope specific to DBC1 immobilized on solid support. The epitope recognized by A300-433A maps to a region between residues 650 and 700 of human Deleted in Breast Cancer 1 using the numbering given in TrEMBL entry Q6P0Q9 (GeneID 57805). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, DBC.1, DBC1, DBC-1, DBIRD complex subunit KIAA1967, deleted in breast cancer 1, Deleted in breast cancer gene 1 protein, KIAA1967, NET35, p30 DBC, p30 DBC
between 650 and 700
2 - 8°C
Detection of human and mouse DBC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DBC1 antibody A300-433A (lot A300-433A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DBC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DBC1 antibody A300-433A (lot A300-433A-2) used for IP at 6 µg per reaction. DBC1 was also immunoprecipitated by a previous lot of this antibody (lot A300-433A-1) and rabbit anti-DBC1 antibody A300-434A For blotting immunoprecipitated DBC1, A300-433A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.