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Rabbit anti-Aurora B Antibody, Affinity Purified - A300-431A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Aurora B Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-431A
Name:
Rabbit anti-Aurora B Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Aurora B immobilized on solid support. The epitope recognized by A300-431A maps to a region between residue 300 and the C-terminus (residue 344) of human Aurora B kinase using the numbering given in Swiss-Prot entry Q96GD4 (GeneID 9212).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, ICC

Product Type:
Antibodies
Alternative Names:
AIK2, AIM1, AIM-1, ARK2, ARK-2, AurB, aurkb-sv1, aurkb-sv2, Aurora 1, aurora- and Ipl1-like midbody-associated protein 1, aurora kinase B, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora/IPL1-related kinase 2, aurora-1, aurora-B, aurora-related kinase 2,
Species Reactivity:
Human
Immunogen:
Between 300 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Aurora B by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa cells treated with nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Aurora B antibody A300-431A (lot A300-431A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Aurora B by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody A300-431A (Lot. No. A300-431A- 2 ) used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Aurora B by immunofluorescence. Samples: Asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody BL1075 (Cat. No. A300-431A) used at 0.5 µg/ml in A and at 2 µg/ml in B. Detection: Secondary antibodies were anti-rabbit IgG conjugated to FITC in A and conjugated to Texas Red in B.

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