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Rabbit anti-TPX2 Antibody, Affinity Purified

Catalogue number:
A300-430A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to TPX2 immobilized on solid support. The epitope recognized by A300-430A maps to a region between residue 700 and the C-terminus (residue 749) of human TPX2 using the numbering given in Swiss-Prot entry Q9ULW0 (GeneID 22974). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

C20orf1, C20orf2, differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in lung cells, DIL2, DIL-2, FLS353, GD:C20orf1, HCA519, HCTP4, hepatocellular carcinoma-associated antigen 519, hepatocellular carcinoma-assoc

Species Reactivity:

Human

Immunogen:

between 700 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human TPX2 by western blot

Detection of human TPX2 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL; 5, 15, 50 µg for A; 50 µg for Input and 1 mg for IP in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-TPX2 antibody BL1679 (Cat. No. A300-430A) used at 0.1 µg/ml for WB (A &B) and at 0.4 µg/mg lysate for IP (B). TPX2 was also immunoprecipitated using rabbit anti-TPX2 antibody BL1677 (Cat. No. A300-429A) at 0.4 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 1 minute.