Antibody was affinity purified using an epitope specific to TPX2 immobilized on solid support. The epitope recognized by A300-430A maps to a region between residue 700 and the C-terminus (residue 749) of human TPX2 using the numbering given in Swiss-Prot entry Q9ULW0 (GeneID 22974). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C20orf1, C20orf2, differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in lung cells, DIL2, DIL-2, FLS353, GD:C20orf1, HCA519, HCTP4, hepatocellular carcinoma-associated antigen 519, hepatocellular carcinoma-assoc
between 700 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human TPX2 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL; 5, 15, 50 µg for A; 50 µg for Input and 1 mg for IP in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-TPX2 antibody BL1679 (Cat. No. A300-430A) used at 0.1 µg/ml for WB (A &B) and at 0.4 µg/mg lysate for IP (B). TPX2 was also immunoprecipitated using rabbit anti-TPX2 antibody BL1677 (Cat. No. A300-429A) at 0.4 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 1 minute.