Antibody was affinity purified using an epitope specific to Cul7 immobilized on solid support. The epitope recognized by A300-428A maps to a region between residue 1650 and the C-terminus (residue 1698) of human Cullin homolog 7 using the numbering given in SwissProt entry Q14999 (GeneID 9820). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
3M1, CUL-7, cullin-7, dJ20C7.5, KIAA0076
between 1650 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse Cul7 by immunoprecipitation. Samples: Whole cell lysate (1 mg) from human U2OS (H) and mouse wild-type MEF (M) cells. Antibody: Affinity purified rabbit anti-Cul7 antibody (Cat. No. A300-428A) used at 3 µg/mg lysate for IP. Western blot of immunoprecipitates was performed using rabbit anti-Cul7 antibody (Cat. No. A300-223A) at 0.33 µg/ml. Detection: Chemiluminescence with an exposure time of 15 seconds.