Antibody was affinity purified using an epitope specific to IRF3 immobilized on solid support. The epitope recognized by A300-426A maps to a region between residues 100 and 150 of human Interferon Regulatory Factor 3 using the numbering given in Swiss-Prot entry Q14653 (GeneID 3661). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
IIAE7, interferon regulatory factor 3, IRF-3
between 100 and 150
2 - 8°C
Detection of human IRF3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IRF3 antibody A300-426A (lot A300-426A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human IRF3 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-IRF3 (Cat. No. A300-426A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB