Antibody was affinity purified using an epitope specific to CRSP3 immobilized on solid support. The epitope recognized by A300-425A maps to a region between residues 1300 and the C-terminus (residue 1368) of human Cofactor Required for Sp1 transcriptional activation, subunit 3 using the numbering given in Swiss-Prot entry Q9ULK4 (GeneID 9439). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
130 kDa transcriptional co-activator, 133 kDa transcriptional co-activator, activator-recruited cofactor 130 kDa component, ARC130, cofactor required for Sp1 transcriptional activation subunit 3, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DRIP130, h
between 1300 and C-term
2 - 8°C
Detection of human CRSP3 by western blot. Samples: Nuclear extract (5 and 25 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-CRSP3 antibody (Cat. No. A300-425A) used at 0.33 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.