Antibody was affinity purified using an epitope specific to CRSP3 immobilized on solid support. The epitope recognized by A300-423A maps to a region between residues 1 and 50 of human Cofactor Required for Sp1 transcriptional activation, subunit 3 using the numbering given in Swiss-Prot entry Q9ULK4 (GeneID 9439). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
130 kDa transcriptional co-activator, 133 kDa transcriptional co-activator, activator-recruited cofactor 130 kDa component, ARC130, cofactor required for Sp1 transcriptional activation subunit 3, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DRIP130, h
between 1 and 50
2 - 8°C
Detection of human CRSP3 by immunoprecipitation. Samples: Nuclear extract (5 mg) from HeLa cells. Antibody: Affinity purified rabbit anti-CRSP3 antibody (Cat. No. A300-423A) used at 4 µg/5 mg extract. Detection: Coomassie stain. The identity of the band that is CRSP3 was verified by mass spectrometry.