Antibody was affinity purified using an epitope specific to CARM1 immobilized on solid support. The epitope recognized by A300-421A maps to a region between residue 550 and the C-terminus (residue 585) of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Coactivator-associated arginine methyltransferase 1, histone-arginine methyltransferase CARM1, PRMT4, protein arginine N-methyltransferase 4
Between 550 and C-term
2 - 8°C
Detection of human and mouse CARM1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody A300-421A (lot A300-421A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody A300-421A (lot A300-421A-3) used for IP at 6 µg per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (A300-421A-2) and rabbit anti-CARM1 antibody A300-420A. For blotting immunoprecipitated CARM1, A300-421A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.