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Rabbit anti-CARM1 Antibody, Affinity Purified - A300-420A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-CARM1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-420A
Name:
Rabbit anti-CARM1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to CARM1 immobilized on solid support.The epitope recognized by A300-420A maps to a region between residues 450 and 500 of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
Coactivator-associated arginine methyltransferase 1, histone-arginine methyltransferase CARM1, PRMT4, protein arginine N-methyltransferase 4
Species Reactivity:
Human, Mouse
Immunogen:
Between 450 and 500
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse CARM1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody A300-420A (lot A300-420A-1) used for WB at 0.06 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human CARM1 by western blot and immunoprecipitation. Sample: Whole cell lysate (25 µg for WB in A; 5 mg for IP in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CARM1 antibody (Cat. No. A300-420A) used at 0.1 µg/ml for WB and at 4 µg/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 1 minute in A. Coomassie stain in B. The identity of the band that is CARM1 was verified by mass spectrometry.

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Detection of human CARM1/PRMT4 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-CARM1/PRMT4 (Cat. No. A300-420A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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