www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-EWS Antibody, Affinity Purified

Catalogue number:
A300-417A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to EWS immobilized on solid support. The epitope recognized by A300-417A maps to a region between residues 100 and 150 of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

bK984G1.4, Ewing sarcoma breakpoint region 1, Ewing sarcoma breakpoint region 1 protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, EWS, EWS oncogene, EWS RNA-binding protein variant 6, EWS-FLI1, RNA-binding protein EWS

Species Reactivity:

Human, Mouse

Immunogen:

between 100 and 150

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse EWS by west

Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody A300-417A (lot A300-417A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human EWS by western blot o

Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody A300-417A (lot A300-417A-2) used for IP at 3 µg per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot A300-417A-1). For blotting immunoprecipitated EWS, A300-417A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human EWS by immunohistoche

Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (Cat. No. A300-417A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB