Antibody was affinity purified using an epitope specific to EWS immobilized on solid support. The epitope recognized by A300-417A maps to a region between residues 100 and 150 of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bK984G1.4, Ewing sarcoma breakpoint region 1, Ewing sarcoma breakpoint region 1 protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, EWS, EWS oncogene, EWS RNA-binding protein variant 6, EWS-FLI1, RNA-binding protein EWS
between 100 and 150
2 - 8°C
Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody A300-417A (lot A300-417A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody A300-417A (lot A300-417A-2) used for IP at 3 µg per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot A300-417A-1). For blotting immunoprecipitated EWS, A300-417A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.