Antibody was affinity purified using an epitope specific to STAT6 immobilized on solid support. The epitope recognized by A300-415A maps to a region between residue 600 and 650 of human Signal Transducer and Activator of Transcription 6 using the numbering given in entry NP_003144.3 (GeneID 6778). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
D12S1644, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT
between 600 and 650
2 - 8°C
Detection of human and mouse STAT6 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (50µg), and mouse NIH 3T3 (15 and 50 µg) cells. Antibodies: Affinity purified rabbit anti-STAT6 antibody A300-415A (lot A300-415A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human STAT6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-STAT6 antibody A300-415A (lot A300-415A-2) used for IP at 6 µg/mg lysate. STAT6 was also immunoprecipitated by a previous lot (A300-415A-1) of this antibody. For blotting immunoprecipitated STAT6, A300-415A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.