Antibody was affinity purified using an epitope specific to TRPM2 immobilized on solid support. The epitope recognized by A300-413A maps to a region between residue 1200 and 1250 of human Transient Receptor Potential Cation Channel, Subfamily M, Member 2 using the numbering given in entry NP_003298.1 (GeneID 7226). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
EREG1, estrogen-responsive element-associated gene 1 protein, KNP3, long transient receptor potential channel 2, LTRPC2, LTrpC-2, NUDT9H, NUDT9L1, transient receptor potential cation channel subfamily M member 2, transient receptor potential channel 7, tr
between 1200 and 1250
2 - 8°C
Detection of human TRPM2 by western blot. Samples: Membrane Prep (50 µg) from Jurkat cells. Antibody: Affinity purified rabbit anti-TRPM2 antibody A300-413A (lot A300-413A-2) used for WB at 2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes
Detection of human TRPM2 by western blot of immunoprecipitates. Samples: Membrane Prep (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody A300-413A (lot A300-413A-2) used for IP at 6 µg per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody A300-414A. For blotting immunoprecipitated TRPM2, A300-414A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.