Rabbit anti-ASC2 Antibody, Affinity Purified - A300-411A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to ASC2 immobilized on solid support. The epitope recognized by A300-411A maps to a region between residue 2000 and the C-terminus (residue 2063) of human Activating Signal Cointegrator-2 using the numbering given in Swiss-Prot entry Q14686 (GeneID 23054). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human ASC2 by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-ASC2 antibody A300-411A (lot A300-411A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human ASC2 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ASC2 antibody A300-411A (lot A300-411A-2) used for IP at 6 µg per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody A300-411A-1. For blotting immunoprecipitated ASC2, A300-411A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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