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Rabbit anti-TBLR1 Antibody, Affinity Purified - A300-408A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-TBLR1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-408A
Name:
Rabbit anti-TBLR1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to TBLR1 immobilized on solid support. The epitope recognized by A300-408A maps to a region between residues 100 and 150 of human TBL1-related Protein 1 using the numbering given in SwissProt entry Q9BZK7 (GeneID 79718).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
C21, DC42, F-box-like/WD repeat-containing protein TBL1XR1, IRA1, MRD41, nuclear receptor co-repressor/HDAC3 complex subunit, nuclear receptor corepressor/HDAC3 complex subunit TBLR1, TBL1-related protein 1, TBLR1, Transducin beta-like 1X-related protein
Species Reactivity:
Human, Mouse
Immunogen:
Between 100 and 150
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse TBLR1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBLR1 antibody A300-408A (lot A300-408A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human TBLR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBLR1 antibody A300-408A (lot A300-408A-2) used for IP at 6 µg per reaction. TBLR1 was also immunoprecipitated by a previous lot of this antibody (lot A300-408A-1). For blotting immunoprecipitated TBLR1, A300-408A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human TBLR1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- TBLR1 (Cat. No. A300-408A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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