Antibody was affinity purified using an epitope specific to Reticulocalbin-1 immobilized on solid support. The epitope recognized by A300-407A maps to a region between residues 1 and 75 of human Reticulocalbin-1, EF-hand calcium binding domain using the numbering given in Swiss-Prot entry Q15293 (GeneID 5954). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory protein Li 84, HEL-S-84, PIG20, proliferation-inducing gene 20, RCAL, RCN, reticulocalbin 1, EF-hand calcium binding domain, reticulocalbin-1
between 1 and 75
2 - 8°C
Detection of human RCN1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5 and 15 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCN1 antibody A300-407A (Lot A300-407A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RCN1 was also immunoprecipitated by rabbit anti-RCN1 antibody lot A300-407A-1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human RCN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RCN1 (Cat. No. A300-407A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB