Antibody was affinity purified using an epitope specific to SERCA2 immobilized on solid support. The epitope recognized by A300-406A maps to a region between residue 475 and 525 of human ATPase, Ca++ transporter, cardiac muscle, slow twitch 2 using the numbering given in entry NP_733765.1 (GeneID 488). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP2B, ATPase Ca++ transporting cardiac muscle slow twitch 2, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, calcium pump 2, calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, cardiac Ca2+ ATPase, DAR, DD
between 475 and 525
2 - 8°C
Detection of human and mouse SERCA2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SERCA2 antibody A300-406A (lot A300-406A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human SERCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SERCA2 antibody A300-406A (lot A300-406A-4) used for IP at 6 µg per reaction. SERCA2 was also immunoprecipitated by a previous lot of this antibody A300-406A-3. For blotting immunoprecipitated SERCA2 , A300-406A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.