Rabbit anti-CDK7 Antibody, Affinity Purified - A300-405A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to CDK7 immobilized on solid support. The epitope recognized by A300-405A maps to a region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7 using the numbering given in Swiss-Prot entry P50613 (GeneID 1022). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC, ICC
Detection of human CDK7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK7 antibody A300-405A (lot A300-405A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of mouse and rat CDK7 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody A300-405A (lot A300-405A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK7 antibody A300-405A (lot A300-405A-2) used for IP at 6 µg per reaction. CDK7 was also immunoprecipitated by a previous lot of this antibody A300-405A-1 For blotting immunoprecipitated CDK7, A300-405A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CDK7 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDK7 (Cat. No. A300-405A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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