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Rabbit anti-G6PD Antibody, Affinity Purified

Catalogue number:
A300-404A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to G6PD immobilized on solid support. The epitope recognized by A300-404A maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

epididymis secretory sperm binding protein, G6PD, G6PD1, glucose-6-phosphate 1-dehydrogenase

Species Reactivity:

Human, Mouse

Immunogen:

between 50 and 100

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse G6PD by wes

Detection of human and mouse G6PD by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody A300-404A (lot A300-404A-4) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse G6PD by western blot

Detection of mouse G6PD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G6PD antibody A300-404A (lot A300-404A-4) used for IP at 6 µg per reaction. G6PD was also immunoprecipitated by a previous lot of this antibody (lot A300-404A-3). For blotting immunoprecipitated G6PD, A300-404A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.