Antibody was affinity purified using an epitope specific to LOK immobilized on solid support. The epitope recognized by A300-399A maps to a region between residues 350 and 400 of human Lymphocyte Oriented Kinase (Serine/Threonine Kinase 10) using the numbering given in TrEMBL entry Q6NSK0 (GeneID 6793). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
LOK, lymphocyte-oriented kinase, PRO2729, serine/threonine-protein kinase 10
between 350 and 400
2 - 8°C
Detection of human LOK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-LOK antibody A300-399A (lot A300-399A-2) used for IP at 6 µg/mg lysate. LOK was also immunoprecipitated by rabbit anti-LOK antibody A300-400A. For blotting immunoprecipitated LOK, A300-399A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.