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Rabbit anti-LOK Antibody, Affinity Purified

Catalogue number:
A300-399A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to LOK immobilized on solid support. The epitope recognized by A300-399A maps to a region between residues 350 and 400 of human Lymphocyte Oriented Kinase (Serine/Threonine Kinase 10) using the numbering given in TrEMBL entry Q6NSK0 (GeneID 6793). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

LOK, lymphocyte-oriented kinase, PRO2729, serine/threonine-protein kinase 10

Species Reactivity:

Human

Immunogen:

between 350 and 400

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human LOK by western blot.

Detection of human LOK by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-LOK antibody A300-399A (lot A300-399A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human LOK by western blot o

Detection of human LOK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-LOK antibody A300-399A (lot A300-399A-2) used for IP at 6 µg/mg lysate. LOK was also immunoprecipitated by rabbit anti-LOK antibody A300-400A. For blotting immunoprecipitated LOK, A300-399A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.