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Rabbit anti-Nanog Antibody, Affinity Purified - A300-398A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Nanog Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-398A
Name:
Rabbit anti-Nanog Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Nanog immobilized on solid support. The epitope recognized by A300-398A maps to a region between residue 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
2410002E02Rik, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, ecat4, ENK, ES cell-associated protein 4, homeobox protein NANOG, homeobox transcription factor Nanog
Species Reactivity:
Mouse
Immunogen:
Between 250 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of mouse Nanog by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from mouse F9 cells. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-398A (lot A300-398A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Nanog was also immunoprecipitated by a previous lot (lot A300-398A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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