Antibody was affinity purified using an epitope specific to Nanog immobilized on solid support. The epitope recognized by A300-398A maps to a region between residue 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
2410002E02Rik, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, ecat4, ENK, ES cell-associated protein 4, homeobox protein NANOG, homeobox transcription factor Nanog
Between 250 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of mouse Nanog by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from mouse F9 cells. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-398A (lot A300-398A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Nanog was also immunoprecipitated by a previous lot (lot A300-398A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).